Bioreactor Off-Gas Monitoring of O2 and CO2

Based on the Oxigraf Model O2Cap Oxygen and CO2 analyzer, the O2Bio is specifically designed to provide gas monitoring of Bioreactor headspace and off-gas streams. The unit will measure continuously from an off-gas vent or periodically via a gas sample valve to take small 10-15ml samples from the bioreactor headspace via keypad or digital interface control.

The O2Bio integrates an Oxigraf tunable diode laser (TDL) oxygen sensor and a non-dispersive infrared (NDIR) CO2 sensor with a pump regulated gas sampling system. The analyzer features a bright vacuum fluorescent alphanumeric display (VFD), touch panel keypad, a rear 0-1VDC analog BNC output for O2 readings, rear terminal strip with limit detection relays and 4 – 20 mA analog outputs of O2 and CO2 reading, and a RS232 digital interface.

Unit of Measure

Features & Applications

Features & Applications

  • Internal Sampling Pump and Solenoid Sample Valve.
  • Electronic flow meter and display.
  • Calibration electronically using 10%O2/5% CO2 and 100% O2 calibration gases.
  • Fast Response (< 1 sec) for prompt analysis.
  • Pressure and temperature corrected.
  • No false oxygen readings with Ar, H2O, CO2, CO, or hydrocarbons.
  • Measures at low flows of 10 to 250 ml/min, conserving overlay gas or via Sample Valve.
  • Relay contacts can be used to control external equipment.
  • Relay contacts are Form C (SPDT), 5 A 250 VAC rating.
  • Analog 4 to 20 mA, analog 0 to 1 VDC, and digital RS-232 outputs.
  • Visual, audible, and remote alarms for Low O2, High O2, Low Flow and System Check.

Performance Conditions


N/A 0 to 10% CO2 (30% CO2 range is optional) 5 to 100% Oxygen


N/A 0.1% in 5 to 100% range (O2), 0.01% in 0 to 10% ranges (CO2)

Sample Flow

N/A 50-250 ml/min with sampling pump on, 50-500ml/min with pump off. Sample Valve control allows gas to flow for a set number of seconds and HOLD that value for periodic measurement.

Response Time

N/A 150ms at 150 ml/min, filter setting to 3.

Stability (4 hrs)

N/A ±0.1% CO2 after 5 minute warm up ±0.1% in Oxygen LN mode ±0.3% in Oxygen XC mode

Dimensions (W x H x D)

N/A 7.5 x 3.0 x 14.0 in190 x 76 x 356 mm

Weight Instrument

N/A 5 Pound2.3 Kilogram

Weight Power Module

N/A 0.5 Pound0.2 Kilogram

Performance Specifications

Ambient Temperature (Operating)

N/A 5 to 40 ºC-2 to 140 ºF

Ambient Temperature (Storage)

N/A -20 to 60 ºC-2 to 140 ºF

Cell Pressure

N/A 10.2 to 17.4 psi70 to 120 kPascal500 to 900 mmHg

Warm-up for Full Accuracy

N/A 5 min

Room Humidity

N/A 0 to 95%, non-condensing

Electrical Specifications

Sensor Power Requirements

N/A 1.5A, 12VDC

External Power Supply

N/A 47 to 63 Hz, 95 to 250 VAC